张灵玲
博士、教授、博士生导师
福建农林大学生命科学学院生物技术系
电话:0591-8378925813314951215
电子信箱:lingling00264@163.com
中国农业生物技术学会微生物生物技术分会理事、中华预防医学会媒介生物学及控制分会蚊虫学组委员,中国生物化学与分子生物学学会农业生物化学与分子生物学分会理事,中国昆虫学会医学昆虫专业委委员,福建省预防医学会消杀灭副主委、《农业生物技术学报》青年编委。福建省科技进步二等奖(第1)、国家科技进步奖二等奖(第8)、福建省青年科技奖、运盛青年科技奖及福建省“五四青年奖章”获得者,福建省百千万人才工程省级人选,福建省高层次C类人才。入选福建省高校杰出青年科研人才计划、福建省新世纪人才、福建农林大学教学名师、拔尖人才及福建农林大学第二届“严家显最高奖教金”。2013.8-2014.8美国密苏里大学访问学者。1999年起从事杀蚊微生物资源筛选、杀蚊机理及应用研究。主持国家自然科学青年基金、教育部博士点青年基金、福建省高校杰出青年科研人才计划等课题。以第1发明人获得授权国家发明专利7项,国际专利1项。以第1/通讯作者发表SCI收录论文32篇。主讲的课程《生物化学B》为国家一流本科课程;主编的《生物化学》教材2022年出版于科学出版社,并入选国家林草十四五规划教材和科学出版社十四五规划教材。指导的本科生连续四届(第十二至十五届)获得全国大学生“挑战杯”竞赛一/二等奖及福建省大学生“挑战杯”竞赛特/一等奖。
发表文章(第1/通讯作者)
Yile Zhang, Caixia Chen, Xi Du, Zhen Yu, Qingqing Min, Chunmei Chen, Haonan Wu, Weilong Tan, Xiong Guan and Lingling Zhang. Urea cycle of Bacillus thuringiensis affects its survival under UV stress. Journal of Agricultural and Food Chemistry. 2024, doi.org/10.1021/acs.jafc.4c00167
Xie Qinghua, Zhu Changqiang, Ai Lele, Nie Danyue, Wu Yifan, Wang Chongcai, He Ji, Tan Weilong, and Zhang Lingling. Epidemiology and genomic characteristics of arenavirus in rodents from the southeast coast of P.R. China. BMC Veterinary Research. 2023, 19:253
Xi Du, Yile Zhang, Haonan Wu, Weilong Tan, Enjiong Huang, Xiong Guan* and Lingling Zhang*. ROS-mediated TCA cycle is greatly related to the UV resistance of Bacillus thuringiensis. Pesticide Biochemistry and Physiology. 2023, 193: 105429
Weibo Ma, Xiong Guan, Ying Miao and Lingling Zhang*. Whole Genome Resequencing Revealed the Effect of Helicase yqhH Gene on Regulating Bacillus thuringiensis LLP29 against Ultraviolet Radiation Stress. International Journal of Molecular Sciences. 2023, 24, 5810.
Xihua Liu, Yile Zhang, Xi Du, Xingyu Luo, Weilong Tan, Xiong Guan and Lingling Zhang*. Effect of yhfS gene on Bt LLP29 antioxidant and UV ray resistance. Pest Management Science. 2023, 79(6): 2087-2097
Alam I, Batool K, Idris AL, Tan WL, Guan X and Zhang LL*. Function of CTLGA9 amino acid residue Leucine-6 in modulation Cry toxicity. Frontiers in Immunology. 2022,13:906259(2022.7.5)
Alam I, Batool K, Idris AL, Tan WL, Guan X and Zhang LL*. Role of Lectin in the response of Aedes aegypti against Bt toxin. Frontiers in Immunology. 2022, 13, 898198(2022.5.13)
Wang JX, Wang XZ, He H, Chen JR, Liu YY, Huang WT, Ou LR, Yang ZH, Guan X, Zhang LL* and Wu SQ*. Knockout of two-Cry-binding Aminopeptidase N isoforms does not change susceptibility of Aedes aegypti larvae to Bacillus thuringiensis subsp. israelensis Cry4Ba and Cry11Aa toxins. Insects. 2021, 12:223(2021.3.5)
Xu J*, Wu CX*, Yang ZH*, Liu WC, Chen H, Batool K, Yao JM, Fan X, Wu J, Rao WH, Huang TP, Xu L, Guan X and Zhang LL*. recG is involved with the resistance of Bt to UV. Pesticide Biochemistry and Physiology. 2020, 167: 104599(2020.5.11)
Ma WB*, Chen HC*, Jiang XY*, Wang JX, Gelbic I, Guan X and Zhang LL*. Whole genome sequence analysis of the mosquitocidal Bacillus thuringensis LLP29. Archives of Microbiology. 2020, 202:1693-1700 (2020.7.29)
Xiaohua Hu, Hong Chen, Jin Xu, Guohui Zhao, Xianhui Huang, Jiannan Liu, Khadija Batool, Chenxu Wu, Songqing Wu, Enjiong Huang, Juan Wu, Munmun Chowhury, Jie Zhang, Xiong Guan, Xiaoqiang Yu, Lingling Zhang. Function of Aedes aegypti galectin-6 in modulation of Cry11Aa toxicity. Pesticide Biochemistry and Physiology. 2020, 162, 96-104 (2019.10.21)
Khadija Batool, Intikhab Alam, Liang Jin, Jin Xu, Chenxu Wu, Junxiang Wang, Enjiong Huang, Xiong Guan, Xiaoqiang Yu and Lingling Zhang. CTLGA9 interacts with ALP1 and APN receptors to modulate Cry11Aa toxicity inAedes aegypti. j. Agric. Food Chem. 2019,67(32):8896-8904(2019.7.24)
Guo-hui Zhao ,Jian-nan Liu , Xiao-hua Hu, Khadija Batool, Liang Jin, Cheng-xu Wu, Juan Wu, Hong Chen, Xiao-yan Jiang, Zhao-hui Yang, Xian-hui Huang, En-jiong Huang, Xiao-Qiang Yu, Xiong Guan and Ling-ling Zhang. Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensistoxicity modulation against Aedes aegypti. Parasites & Vectors, 2019, 2:319(2019.6.25)
Lingling Zhang (#), Xiaohua Hu, Songqing Wu, Kahdija Batool, Munmun Chowdhury, Yi Lin, Zhang J, Sarjeet S. Gill, Xiong Guan (*) and Xiaoqiang Yu (*). 2018. Aedes aegyptiGalectin competes with Cry11Aa for binding to ALP1 to modulate Cry toxicity, Journal of Agricultural and Food Chemistry, 66(51):13435-13443(2018.12.17)
Lingling Zhang(#), Xiaojuan Zhang, Khadija Batool, Xiaohua Hu, Mingfeng Chen, Jin Xu, Junxiang Wang, Xiaohong Pan, Tianpei Huang, Lei Xu, Xiaoqiang Yu(*) and Xiong Guan (*). 2018.Comparison and mechanism of the UV-resistant mosquitocidal Bt mutant LLP29-M19, Journal of Medical Entomology, 55(1):210-216(2017.11.23)
Khadija Batool, Intikhab Alam, Guohui Zhao, Junxiang Wang, Jin Xu, Xiaoqiang Yu, Enjiong Huang, Xiong Guan andLingling Zhang. 2018. C-type Lectin-20 interacts with ALP1 receptor to reduce Cry toxicity inAedes aegypti.Toxins. 10: 390(2018.10.11)
Khadija Batool, Intikhab Alam, Songqing Wu, Wencheng Liu, Guohui Zhao, Mingfeng Chen, Junxiang Wang, Jin Xu, Tianpei Huang, Xiaohong Pan, Xiaoqiang Yu,Xiong Guan,Lei Xu &Lingling Zhang (*). 2018.Transcriptomic Analysis of Aedes aegypti in Response to MosquitocidalBacillus thuringiensis LLP29 Toxin.ScientificReports,8:12650 (2018.8.23)
Lingling Zhang (#), Guohui Zhao, Xiaohua Hu, Jiannan Liu, Mingwei Li, Kahdija Batool, Mingfeng Chen, Junxiang Wang, Jin Xu, Tianpei Huang, Xiaohong Pan, Lei Xu, Xiaoqiang Yu(*) and Xiong Guan(*),Cry11Aa interacts with the ATP-Binding protein fromCulex quinquefasciatusto improve the toxicity, Journal of Agricultural and Food Chemistry, 2017, 65:10884-10890(2017.12.7)
Ensi Shao, Guifang Lin, Sijun Liu (*), Xiaoli Ma, Ming-feng Chen, Li Lin, Sonqing Wu, Li Sha, Zhaoxia Liu, Xiaohua Hu, Xiong Guan,Lingling Zhang(*). 2017. Identification of transcripts involved indigestion, detoxification and immune response from transcriptome ofEmpoasca vitis( Hemiptera: Cicadellidae) nymphs. Genomics, 109(1):58-66(2016.11.17)
Qiuqiu Lin, Pengli Zhu, Rebeca Carballar-Lejarazu, Ivan Gelbic, Xiong Guan, Lei Xu(*),Lingling Zhang (*). 2017. The colonization ofBacillus thuringiensisstrains in bryophytes. Turkish Journal of Biology, 41: 41-48(2017.1.1
Xiaohua Hu, Yajie Guo,Songqing Wu, Zhaoxia Liu, Tao Fu, Ensi Shao, Carballar-Lejarazú Rebeca,Guohui Zhao,Zhipeng Huang, Ivan Gelbič, Xiong Guan, Shuangquan Zou, Lei Xu(*), and Lingling Zhang (*). 2017.Effect of proteolytic and detoxification enzyme inhibitors on Bacillus thuringiensis var.israelensis tolerance in the mosquito Aedes aegypti. Biocontrol Science & Technology, 27(2):169-179(2017.2.17)
Lingling Zhang(#), Xiaojuan Zhang, Yi Zhang, Songqin Wu, Ivan Gelbic, Lei Xu, Xiong Guan(*). 2016.A new formulation ofBacillus thuringiensis: UV protection and sustained release mosquito larvae studies, scientific report,6: 39425
Songqing Wu, Yan Peng, Zhangmin Huang, Zhipeng Huang, Lei Xu, Ivan Gelbič, Xiong Guan,Lingling Zhang (*), and Shuangquan Zou (*). 2016. Isolation and characterization of a novel nativeBacillus thuringiensis strain BRC-HZM2 capable of degrading chlorpyrifos, Journal of Basic Microbiology,26: 227-238
Songqing Wu, Wei Wu, Xiaoli Zhu, Zhaoxia Liu, Carballar-Lejarazú Rebeca, Tao Fu, Chaoqiang Han, Ensi Shao, Xiaohong Pan, Zhipeng Huang, Lei Xu, Shuangquan Zou,Lingling Zhang(*) & Xiong Guan(*). 2016.Physiological and biochemical response of Aedes aegypti tolerance to Bacillus thuringiensis. Biocontrol Science and Technology, 26: 227-238
Lingling Zhang(#), Jie Yu, Yufei Xie, Hongli Lin, Zhipeng Huang, Lei Xu, Ivan Gelbičand Xiong Guan (*), Biological activity ofBacillus thuringiensis chitinase against Caenorhabditis elegans. Journal of Economic Entomology, 2014, 107(2):551-558
Songqing Wu, Yanjiao Lan, Dongmei Huang, Yan Peng, Zhipeng Huang, Lei Xu, Ivan, GelbičRebeca Carballar-Lejarazu, Xiong Guan,Lingling Zhang(*), and Shuangquan Zou (*), Use of spent mushroom substrate for production ofBacillus thuringiensis by solid-state fermentation, J Econ Entomol.,2014, 107(1):137-143
Lingling Zhang (#), Baozhen Tang, Eenjiong Huang, Zhipeng Huang, Zhaoxia Liu, Tianpei Huang, Ivan Gelbič and Xiong Guan (*), Different toxicity of the novelBacillus thuringiensis strain LLP29 againstAedes albopictus andCulex quinquefasciatus, J Econ Entomol., 2013, 160 (2): 1098-1102
Lingling Zhang (#), Sixin Qiu, Tianpei Huang, Zhipeng Huang, Lei Xu, Changbiao Wu, Ivan Gelbičand Xiong Guan (*), Effect of chemical additives onBacillus thuringiensisagainstPlutella xylostella, J Econ Entomol, 2013, 160 (3): 1075-1080
Songqing Wu, Yanjiao Lan, Zhimao Wu, Yan Peng, Siqi Chen, Zhipeng Huang, Lei Xu, Ivan Gelbič, Xiong Guan,Lingling Zhang (*) and Shuangquan Zou (*), Pretreatment of spent mushroom substrate for enhancing the conversion of fermentable sugar, Bioresource Technology, 2013, 148: 596-600
Changbiao Wu, Lili Wu, Lei Xu,Lingling Zhang(*)and Xiong Guan, Parasporal crystal proteins ofBacillus thuringiensis strain BT6, originated from the faecal of Sika Deer, demonstrates anticoccidial activity againstEimeria tenella, African Journal of Microbiology Research, 2013, 7(33):4261-4264
Lingling Zhang (#), Qi Zhang, Enjiong Huang, Mingwei Li, Tianpei Huang, Changbiao Wu, Xiong Guan (*) and Ivan Gelbič (*), A differentially displayed mRNA relates to the resistance ofAedes albopictusselected in vitro-activated Cyt1Aa6, Journal of the America Mosquito Control Association,2012, 28: 327-329
Lingling Zhang (#), Yan Peng, Songqing Wu, Linying Sun, Enjiong Huang, Tianpei Huang, Lei Xu, Changbiao Wu, Ivan Gelbičand Xiong Guan (*), Microbial Ecology and Association ofBacillus thuringiensis in Chicken feces Originating from feed, Current Microbiology, 2012, 65: 784-791
Lingling Zhang (#), Sixin Qiu, Tianpei Huang, Zhipeng Huang, Lei Xu, Changbiao Wu, Ivan Gelbičand Xiong Guan (*), Effect of chemical additives onBacillus thuringiensisagainstPlutella xylostella, J Econ Entomol, 2013, 160 (3): 1075-1080
Lingling Zhang (#), Songqing Wu, Yan Peng, Mingwei Li, Linying Sun, Eenjiong Huang, Xiong Guan and Ivan Gelbic (*), The novel mosquitocidalBacillus thuringiensisstrain LLP29 provides potential for use in practice, Journal of Vector Ecology, 2011, 36(2): 458-460
Lingling Zhang (#), Enjiong Huang, Jin Lin, Ivan Gelbic*, Qunlin Zhang, Yi Guan, Tianpei Huang and Xiong Guan(*),A novel mosquitocidalBacillus thuringiensis strain LLP29 isolated from the Phylloplane ofMagnolia denudata, Microbiological Research,2010,16:133-141
Zhang L (#), Huang E, Gelbič I, Guan C, Guan Y and Guan X. Cloning, expression and characterisation of a promising mosquitocidal gene. Indian Journal of Experimental Biology, 2009, 47: 799-803
Zhang L (#), Lin J, Luo L, Guan C, Zhang Q, Guan Y and Guan X. A novel Bacillus thuringiensis strain LLB6 isolated from bryophyte and its new cry2Ac-type gene. Letters in Applied Microbiology, 2007, 44: 301-307
授权南非专利
申请人: 张灵玲,关雄,黄志鹏,张小娟,吴松青,沙莉,潘晓鸿,黄天培,邵恩斯,黄必旺,陈慧成,朱小丽
专利名称:A mosquitocidal Bacillus thuringiensis suspending sustained preparation and a method of preparing
国际申请号:PCT/CN2014/093158
申报日:2014.12.5 通过国际查新日:2015.2.12,授权日:2017.6.28
授权国家发明专利
A、张灵玲、陈宇熹、关雄、黄天培、叶舒婷、林志艺、蔡美凤、郑毅、吴丽云、吴松青、翁林捷、沙莉,杀蚊苏云金芽孢杆菌液体培养基, 2012.4.18, 中国,ZL201010200543.X。
B、张灵玲、吴松青、彭艳、关雄、张小娟、黄冬梅、陈忍、姚祖杰、张小娟, 一种苏云金杆菌液态发酵培养基的制备方法,2012.12.19,中国,ZL201110258526.6。
C、张灵玲、吴松青、彭艳、关雄、黄冬梅、兰艳娇、洪冬华,一种苏云金杆菌固态发酵培养基的制备方法,2013.5.15,中国,ZL 201110207054.1。
D、张灵玲、许雷、关雄、张永嵘,一种苏云金芽胞杆菌乳化发酵培养基,2013.6.19,中国,ZL 201210278484.7。
E、张灵玲、许雷、关雄、张永嵘,一种苏云金芽胞杆菌糖苷发酵培养基,2013.10.23,中国,CN201210278035.2。
F、张灵玲、邵恩斯、关雄、刘斯军、庄浩瀚、徐碧虹、林莉、林立金,对水稻褐飞虱高毒力的Bt毒素Cry1Ab-loop2-P2S和工程菌,2014.5. 21,中国,201210433476.5。
H、张灵玲,关雄,张小娟,顾晓军,张意,郑晓娟,吴松青,王官栋,吴志卯,刘红,一种杀灭蚊子幼虫的苏云金杆菌制剂及其制备方法,2015.11.18,中国,ZL201310733631.X
I、关雄、黄志鹏、张灵玲
一种生物药肥的制备方法(专利号:ZL 02115602.6)
授权日:2005.5.20
J、高产耐高温蛋白酶的苏云金芽孢杆菌筛选及培养方法
申请人:关雄、郑毅、黄志鹏、黄必旺、黄天培、沙莉、李今煜、张群林、吴昌标、余洁、张灵玲
申请日:2006.10.12 申请号:200610069339.2 初审合格:2006.12.6
奖励
1、2022年福建运盛青年科技奖
2、“重要蚊虫快速鉴定及高效杀蚊Bt制剂创制关键技术”获2019年福建省科技进步二等奖(第1完成人)
3、入选2021年福建省百千万人才工程省级人选
4、2021年第16届福建省青年科技奖
5、 “科教协同育人背景下生物类大学生创新型人才培养模式的构建与实践” 获2021年福建农林大学高等教育教学成果奖二等奖(张灵玲、张惠莹、袁军、吴松青、林文芳、郑燕、沙莉、关雄)
6、2019年校首届“教学名师”、校“教学领军人才”
7、“细菌农药新资源及产业化新技术新工艺研究”获2010年国家科技进步二等奖(第8完成人)
8、2009.2.5 “杀虫防病微生物农药新资源及产业化新技术新工艺研究” 福建省科学技术奖一等奖(第11完成人)
9、“茶园Bt增效剂及药肥技术开发研究”获2005年福建省科学技术奖二等奖 (第12完成人)
10、2012年入选福建省高校杰出青年科研人才计划
11、2013年“严家显最高奖教金”获得者;
12、2014年“福建省五四青年”获得者;
13、2014年获福建农林大学金山学者“拔尖人才”;
14、2014年入选福建省新世纪人才;
15、指导本科生参加全国挑战杯课外作品赛获奖情况
(1)2017年第十五届全国挑战杯课外作品赛二等奖及累进创新铜奖
本科生:郭雅洁、赵国辉、戴晓艺、刘文诚、郑茹萍
项目名称:Bt杀虫增效新物质的挖掘及新型杀线虫剂的制备
指导老师:张灵玲
主办单位:团中央、中国科协、教育部、中国社科院、全国学联、上海人民政府
(2)2015年指导本科生获第十四届全国挑战杯课外作品赛二等奖
本科生:朱小丽, 陈慧成,刘健楠,徐卓吟,郭雅洁
项目名称: Bt高抗紫外突变株的筛选及其漂浮缓释剂制备
指导老师:张灵玲
主办单位:团中央、中国科协、教育部、全国学联、广东省政府
(3)2013年指导本科生获第十三届全国挑战杯课外作品赛二等奖
本科生:刘昭霞,苏伟超,李宁,兰艳娇,洪冬华
项目名称:埃及伊蚊抵御Bt菌株LLP29侵染的转录组分析及其关键基因功能鉴定
指导老师:张灵玲
主办单位:团中央、中国科协、教育部、全国学联、江苏省政府
(4)2011年指导本科生获第十二届全国挑战杯课外作品赛一等奖
本科生:关怡,黄冬梅,张小娟,林菲菲
项目名称:白纹伊蚊高效Bt的筛选及细胞受体蛋白分析
指导老师:张灵玲
主办单位:团中央、中国科协、教育部、全国学联、辽宁省政府
课题:
1、军民联合项目,20SWAQX02, 媒介蚊虫病原微生物研究,2021-2024,14万,在研,主持;
1、福建省自然科学基金,2020J01550,蛋白乙酰化修饰在Bt抵抗紫外线胁迫的作用,2020.8.1-2023.8.1,7万,在研,主持;
2、国家科技部重点研发计划项目,2017YFE0122000,环境友好复合生物杀虫剂:改变中国和英国的农业害虫控制,2019.3-2022.3,476万元,在研,参加;
3、福建省卫生联合攻关计划,2019-WJ-29,埃及伊蚊Lectin对Bt毒素杀蚊的干扰机理及应用,2019.9-2022.9,35万元 (其中:福建农林大学15万、福建省疾病预防控制中心20万),在研,主持;
4、病原微生物生物安全国家重点实验室开放课题,SKLPBS1838,蚊媒埃及伊蚊对苏云金杆菌侵染的应答研究,2018.11-2020.10,20万元,在研,主持;
5、福州市科技局项目,2018-G-70,橄榄主要害虫小黄卷叶蛾的生物防治,2018.03-2021 .02,15万元,在研,主持;
6、福建农林大学创新基金,KFA17315A,假眼小绿叶蝉中肠转录组分析及对Cry毒素的酶解,2018.1-2020.12,22万元,在研,主持;
7、国家自然科学基金,31301724,凝集素参与埃及伊蚊抵御Cry11A毒素过程的分子机理,2014.1-2016.12,22万元,结题,主持;
8、福建省新世纪人才支持计划,JA13089,凝集素在埃及伊蚊抵御Cry11A毒素过程的作用及其机理,2014-2016,3万元,结题,主持;
9、福建省科技计划重点项目,2013N0003,苏云金杆菌防治蕈蚊关键技术及应用研究,2013-2016,10万元,结题,主持;
10、福建省自然科学基金,013J01079,苏云金杆菌叶片分离株LLP29在白玉兰植株上的定位分析,2013-2016,4万元,结题,主持;
11、福建农林大学杰出青年科技人才培养,xjq201203,Bt LLP29侵染埃及伊蚊的应答机制 ,2013.01-2015.12,30万元,结题,主持;
12、福建省高校杰出青年科研人才培育计划,JA12092,2012-2014,2万元,结题,主持
13、教育部博士点青年基金,20093515120010,源自苔藓的Bt分离株细胞学与组织学分析 ,2010-2012,3.6万元,结题,主持;
14、福建青年人才项目,2008F3012,不同来源Bt的分离及高效菌株杀蚊基因的功能分析, 2008-2011,4万元,结题,主持;
15、福建省教育厅项目,JA08080,从白玉兰叶片中分离Bt新资源及高效菌株杀蚊机理研究 ,2008-2011,0.5万元,结题,主持;
16、福建农林大学青年教师科研基金重点项目,08A01,高效杀蚊Bt新菌株LLP29杀蚊机理 的研究,2008-2011,1.5万元,结题,主持